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Preparation for protein isolation; Protein extraction and solubilization; Protein concentration determination; Concentratig protein solutions; Gel electrophoresis under denaturing conditions; Gel electrophoresis under nondenaturing conditions; Isoelectric focusing two-dimensional gel electrophoresis; Immunoblotting; Ion exchange chromatography; Gel filtration chromatography; Affinity chromatography; Hanging drop crystallization.
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